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KMID : 0381019980310040869
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Korean Journal of Nutrition
1998 Volume.31 No. 4 p.869 ~ p.869
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Chung, Keun Hee
Yerokun, Todunbo/Kahn, Akaba/Ringer, David
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Abstract
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A complementary DNA(cDNA) for rat hepatic aryl sulfotransferase MAST IV) was isolated, characterized, and used as a hybridization probe to evaluate the molecular basis for the differential expression of AST IV during 2-acetylaminofluorine (2AAF)-induced hepatocarcinogenesis. The AST IV cDNA clone was obtained by immounochemical screening of a male Sparque-Dawley rat liver cDNA library. The AST IV cDNA was found to be 1.3 kilobases long and to encode a fusion protein which was reactive with an antibody to AST IV and enzymatically able to generate the sulfuric acid ester of N-hydroxy-2AAF. Sequence analysis of the AST IV cDNA showed it to be 1127 residues in length and to have essentially conplete homology with PST-I cDNA, a previously reported, 1028-base cDNA for an uncharacterized rat liver aryl sulfotransferase.
This report contains also the first description of the genomic structure for a sulfotransferase(ST). The gene(ASTIV) encodes rat hepatic aryl ST 1V, also known as tyrosine-ester ST(EC 2.8, 2.9). A phage genomic clone containing 70% of the 3´ AST gene coding sequence was isolated after screening a rat genomic library with an ASTIV cDNA. The remaining 5´ sequence was determined from a PCR product obtained from rat genomic DNA and ASTIV cDNA specific primers. ASTIV spans 3.5kb and contains eight exons and seven introns.
A nutri tion -related study on the role of zinc, copper and cadmium in the regulation of aryl sulfotranserase N activity in the rat liver is contained.
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